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Updated: Mar 10, 2026

Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping
Published on: September 4, 2009
Establishment of human embryonic stem cell line with a complex abnormal karyotype and the stable XIST expression
Leiyu Deng1, Dehua Cheng1, Guangxiu Lu2
1Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
Abstract:
The embryonic stem cell line chHES-3XISTa was derived from heterogeneous chHES-3 cells. chHES-3XISTa showed a new abnormal karyotype of 46,XX with 8 derivation chromosomes and expressed X inactive specific transcript (XIST) in continual culture. Tri-methylation of H3 Lys-27 (H3K27me3) punctate enrichment located in RNA Polymerase II hole was also found in all chHES-3XISTa cells. Pluripotent markers and differentiate capability in vitro were confirmed by immunochemistry staining.
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