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Updated: Mar 10, 2026

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
Published on: September 16, 2019
Deazaguanine derivatives, examples of crosstalk between RNA and DNA modification pathways
Geoffrey Hutinet1, Manal A Swarjo2, Valérie de Crécy-Lagard1
1a Department of Microbiology and Cell Science , University of Florida , Gainesville , FL , USA.
Abstract:
Seven-deazapurine modifications were thought to be highly specific of tRNAs, but have now been discovered in DNA of phages and of phylogenetically diverse bacteria, illustrating the plasticity of these modification pathways. The intermediate 7-cyano-7-deazaguanine (preQ0) is a shared precursor in the pathways leading to the insetion of 7-deazapurine derivatives in both tRNA and DNA. It is also used as an intermediate in the synthesis of secondary metabolites such as toyocamacin. The presence of 7-deazapurine in DNA is proposed to be a protection mechanism against endonucleases. This makes preQ0 a metabolite of underappreaciated but central importance.
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