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Updated: Mar 10, 2026

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
Performance of a MALDI-TOF MS-based imipenem hydrolysis assay incorporating zinc sulfate
1Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, John St, Hawthorn, Melbourne, 3122, Australia; Department of Microbiology, Melbourne Pathology, 103 Victoria Pde, Collingwood, Melbourne, 3066, Australia.
Abstract:
A MALDI-TOF MS1-based imipenem hydrolysis assay was modified by adding ZnSO4. This improved detection of metallo-β-lactamase producing strains without compromising detection of other carbapenemase types. Using 129 genetically characterized Gram-negative bacilli, the sensitivity and specificity were 98.5% (95% confidence interval [CI]: 91.9-99.7%) and 100% (95% CI: 94.3-100%), respectively.
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