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Methacrylate-bonded covalent-organic framework monolithic columns for high performance liquid chromatography
Li-Hua Liu1, Cheng-Xiong Yang1, Xiu-Ping Yan2
1College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin 300071, China.
Journal of Chromatography. A
|December 13, 2016
Summary
Covalent-organic frameworks (COFs) were modified into methacrylate-bonded monolithic columns for high-performance liquid chromatography (HPLC). These COF columns offer improved separation efficiency and interactions for analyzing small molecules.
Area of Science:
- Materials Science
- Analytical Chemistry
- Chromatography
Background:
- Covalent-organic frameworks (COFs) are novel microporous materials with unique properties.
- Traditional COF synthesis yields irregular particles, hindering their use in high-performance liquid chromatography (HPLC).
Purpose of the Study:
- To develop COF-based monolithic columns for HPLC applications.
- To overcome the limitations of traditional COF synthesis for chromatographic separations.
Main Methods:
- Preparation of methacrylate-bonded COF monolithic columns.
- Evaluation of column homogeneity, permeability, and separation performance in HPLC.
- Analysis of small molecules including polycyclic aromatic hydrocarbons, phenols, anilines, nonsteroidal anti-inflammatory drugs, and benzothiophenes.
Main Results:
- The COF bonded monolithic columns exhibited good homogeneity and permeability.
- High column efficiency, resolution, and precision were achieved for small molecule separations.
- Enhanced hydrophobic, π-π, and hydrogen bond interactions were observed in reverse-phase HPLC compared to bare polymer columns.
Conclusions:
- Methacrylate-bonded COF monoliths are effective for HPLC separations.
- COF-based materials show significant potential for advancing separation sciences.
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