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Updated: Mar 10, 2026
Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing
Published on: October 19, 2014
Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles
Sybren L N Maas1,2, Marike L D Broekman1,2, Jeroen de Vrij3,4,5
1Department of Neurosurgery, University Medical Center Utrecht, Utrecht, The Netherlands.
Abstract:
Accurate characterization of extracellular vesicles (EVs), including exosomes and microvesicles, is essential to obtain further knowledge on the biological relevance of EVs. Tunable resistive pulse sensing (tRPS) has shown promise as a method for single particle-based quantification and size profiling of EVs. Here, we describe the technical background of tRPS and its applications for EV characterization. Besides the standard protocol, we describe an alternative protocol, in which samples are spiked with polystyrene beads of known size and concentration. This alternative protocol can be used to overcome some of the challenges of direct EV characterization in biological fluids.
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