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Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
Published on: June 26, 2016
Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR
Shayne A Bellingham1,2, Mitch Shambrook3, Andrew F Hill4
1Department of Biochemistry and Molecular Biology, The University of Melbourne, Melbourne, VIC, Australia.
Abstract:
Extracellular vesicles, such as exosomes and microvesicles, have been shown to contain potential microRNA (miRNA) biomarkers that may be utilized in the diagnosis of various diseases from cancer to neurological disorders. The unique nature of the extracellular vesicle bilayer allows miRNA to be protected from degradation making it an ideal source of material for biomarkers discovery from both fresh and archived samples. Here we describe the quantitative analysis of miRNA isolated from exosomes by quantitative PCR and digital PCR.
