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Isolation of Platelet-Derived Extracellular Vesicles
Maria Aatonen1, Sami Valkonen1,2, Anita Böing2
1Division of Biochemistry and Biotechnology, Faculty of Biological and Environmental Sciences, University of Helsinki, Viikinkaari 5 D, 56, 00014, Helsinki, Finland.
Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2016
Summary
Platelet-derived extracellular vesicles (PDEVs) are crucial in various physiological and pathological processes. This chapter details common methods for isolating these important vesicles from blood and platelets.
Area of Science:
- Hematology
- Cell Biology
- Biochemistry
Background:
- Platelets are vital for hemostasis, immunity, and development.
- Platelets also significantly contribute to arterial thrombosis and cancer progression.
- Platelet-derived extracellular vesicles (PDEVs) are recognized as key mediators and biomarkers due to their diverse functions.
Purpose of the Study:
- To provide a comprehensive overview of PDEV isolation techniques.
- To clarify the origin and terminology of platelet- and megakaryocyte-derived EVs.
- To guide researchers in selecting appropriate methods for PDEV isolation.
Main Methods:
- Isolation of PDEVs from whole blood.
- Isolation of PDEVs from activated, purified platelets.
- Description of commonly employed PDEV isolation protocols.
Main Results:
- Detailed explanation of established PDEV isolation methodologies.
- Clarification that PDEVs encompass EVs from both platelets and megakaryocytes.
- Overview of techniques applicable to different starting materials (blood vs. platelets).
Conclusions:
- Standardized isolation methods are essential for studying PDEVs.
- Understanding PDEV origins and isolation is critical for biomarker and therapeutic research.
- This chapter serves as a practical guide for PDEV isolation from blood and platelets.
