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Updated: Aug 12, 2026

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Published on: March 22, 2012
Components of the human T lymphocyte antigen receptor complex, CD3-p28 and CD3-gamma, are biochemically distinct
1Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN.
Abstract:
A recently described subunit of the T cell antigen receptor-CD3 complex, p28, is very similar to the mature CD3-gamma subunit with respect to electrophoretic mobility. We demonstrated, by one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and endo-beta-n-acetylglucosaminidase digestion, that CD3-p28 and CD3-gamma coexist in CD3-specific immunoprecipitates from the human T lymphocyte hybrid cell line 255.88, which was labeled by biosynthetic incorporation of radioactive amino acids. By partial proteolysis and peptide mapping, we demonstrated that CD3-p28 and CD3-gamma are distinct members of the CD3 complex.
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