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Related Experiment Video

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Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform
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Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis.

Yih Yang Chen1, Pamuditha N Silva1, Abdullah Muhammad Syed1

  • 1Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9.

Proceedings of the National Academy of Sciences of the United States of America
|December 14, 2016
PubMed
Summary
This summary is machine-generated.

We developed a microfluidic system to rapidly convert tissues into optically transparent structures, enabling clearer imaging for drug screening and regenerative medicine applications.

Keywords:
3D imagingCLARITYcomputational analysisfluorescence imagingmicrofluidic

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Microfluidics

Background:

  • Intratissue optical scattering hinders on-chip imaging of 3D tissues in microfluidic devices.
  • This limitation impedes the use of tissue models for high-throughput screening assays.

Purpose of the Study:

  • To engineer a microfluidic system for rapid tissue clearing and optical transparency.
  • To enable advanced imaging of intact tissues for applications in drug screening and regenerative medicine.

Main Methods:

  • Engineered a microfluidic system to preserve and convert tissues into optically transparent structures in under 1 day.
  • Achieved accelerated clearing by enhancing interstitial fluid exchange 567-fold, increasing lipid removal.
  • Developed an image analysis algorithm for automated analysis of vasculature and cellular distribution.

Main Results:

  • The microfluidic system achieved tissue clearing 20x faster than passive methods.
  • Enabled fluorescent imaging and mapping of cell segregation in tumor spheroids.
  • Allowed tracking of vasculature degradation in pancreatic islets, with implications for diabetes research.

Conclusions:

  • The developed technique allows whole tissue analysis in microfluidic systems.
  • Has significant implications for organ-on-a-chip development, high-throughput drug screening, and regenerative medicine.