Related Experiment Videos
Cell proliferation of human bladder tumors
H Tsujihashi1, H Matsuda, S Uejima
1Department of Urology, Kinki University School of Medicine, Osaka, Japan.
The Journal of Urology
|October 1, 1989
Summary
The bromodeoxyuridine (BrdUrd) labeling index (LI) can predict bladder tumor malignancy. Higher BrdUrd LI in bladder tumors correlates with increased potential for malignancy and poorer prognosis, aiding in assessing tumor behavior.
Area of Science:
- Oncology
- Cell Biology
- Biochemistry
Background:
- Bladder tumors exhibit variable cell proliferation rates.
- Accurate assessment of tumor cell proliferation is crucial for predicting prognosis.
- Existing methods for assessing proliferation may have limitations.
Purpose of the Study:
- To evaluate the utility of bromodeoxyuridine (BrdUrd) labeling index (LI) in assessing bladder tumor cell proliferation.
- To correlate BrdUrd LI with tumor grade, invasiveness, and clinical outcomes.
- To determine if BrdUrd LI can serve as a prognostic marker for bladder cancer.
Main Methods:
- Immunohistochemical technique using anti-bromodeoxyuridine (BrdUrd) antibody.
- In vitro labeling of bladder tumor cells during DNA synthesis phase.
- Calculation of the labeling index (LI) as the percentage of labeled S-phase cells.
Main Results:
- Bladder tumors showed a significantly higher average BrdUrd LI (13.0%) compared to normal epithelium (5.0%).
- Higher LI was observed in high-grade, invasive tumors, and those with lymph node involvement.
- Increased LI correlated with tumor recurrence and poorer outcomes in patients undergoing TUR and cystourethrectomy.
Conclusions:
- The BrdUrd labeling index is a reliable method for quantifying bladder tumor cell proliferation.
- Elevated BrdUrd LI indicates a higher malignant potential and poorer prognosis in bladder cancer.
- BrdUrd LI measurement offers valuable insights into the biological behavior of bladder tumors.