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Updated: Mar 10, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
Tandem Mass Spectrometry in Combination with Product Ion Mobility for the Identification of Phospholipids
Karin A Zemski Berry1, Robert M Barkley1, Joseph J Berry2
1Department of Pharmacology, University of Colorado Denver , Mail Stop 8303, 12801 E. 17th Avenue, Aurora, Colorado 80045, United States.
Abstract:
Concerted tandem and traveling wave ion mobility mass spectrometry (CTS analysis) is a unique method that results in a four-dimensional data set including nominal precursor ion mass, product ion mobility, accurate mass of product ion, and ion abundance. This nontargeted lipidomics CTS approach was applied in both positive- and negative-ion mode to phospholipids present in human serum, and the data set was used to evaluate the value of product ion mobility in identifying lipids in a complex mixture. It was determined that the combination of diagnostic product ions and unique collisional cross-section values of product ions is a powerful tool in the structural identification of lipids in a complex biological sample.
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