Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.6K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.6K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.5K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.5K
Cohesins02:20

Cohesins

5.8K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
5.8K
Cleavage and Blastulation01:33

Cleavage and Blastulation

50.9K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
50.9K
Anaphase A and B01:39

Anaphase A and B

5.7K
Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
5.7K
Replicative Cell Senescence02:15

Replicative Cell Senescence

4.5K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial: Innovative field diagnostics for real-time plant pathogen detection and management.

Frontiers in plant science·2026
Same author

Recent Advances on Application of Modified Biochar for the Removal of Pharmaceutical Compounds from Wastewater.

ChemistryOpen·2026
Same author

Thermal and storage degradation kinetics of corn anthocyanin-based natural red colorants in a model beverage system, and their gastrointestinal behavior.

NPJ science of food·2026
Same author

Multigene analysis reveals '<i>Candidatus</i> Phytoplasma asiaticum' (16SrII-C) association with niger and sesame phyllody in Madhya Pradesh, Central India and identification of a putative vector.

Frontiers in plant science·2026
Same author

Street women empowered and engaged to stop TB: A 'mixed method' study.

The Indian journal of tuberculosis·2026
Same author

Therapeutic potential of 6-BIO in modulating oxidative stress and inflammation in rheumatoid arthritis.

Molecular biology reports·2026
Same journal

Zinc Finger Proteins as Regulators of Organ Fibrosis.

Journal of cellular biochemistry·2026
Same journal

Intrinsic Disorder Status in Human Proteins Interacting With SARS-CoV-2 Proteins: Insights From Five Years of Translational Research.

Journal of cellular biochemistry·2026
Same journal

The Effect of Protein Tagging on Aggregation and Phase Separation.

Journal of cellular biochemistry·2026
Same journal

TRIM32 Alleviates the Inflammation in Spinal Cord Injury Progression Through Inducing the Ubiquitination Degradation of TLR4.

Journal of cellular biochemistry·2026
Same journal

Bedaquiline Binding at the Leading Site of Mycobacterium tuberculosis ATP Synthase Induces Distinct Structural and Dynamic Changes.

Journal of cellular biochemistry·2026
Same journal

Agrin Ablation in Osteoblasts Compromises Long Bone Structure and Osteoblastic Differentiation of Mesenchymal Stem Cells.

Journal of cellular biochemistry·2026
See all related articles

Related Experiment Video

Updated: Mar 10, 2026

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
07:05

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Published on: September 8, 2021

2.8K

Separase: Function Beyond Cohesion Cleavage and an Emerging Oncogene.

Ravinder Kumar1

  • 1Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400 076, Maharashtra, India.

Journal of Cellular Biochemistry
|December 15, 2016
PubMed
Summary
This summary is machine-generated.

Accurate chromosome segregation is vital for species survival. This review highlights separase

Keywords:
CENTRIOLECOHESINONCOGENESEPARASE

More Related Videos

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
09:58

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

3.2K
Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays
09:29

Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays

Published on: February 2, 2024

4.1K

Related Experiment Videos

Last Updated: Mar 10, 2026

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
07:05

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Published on: September 8, 2021

2.8K
Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
09:58

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

3.2K
Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays
09:29

Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays

Published on: February 2, 2024

4.1K

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology
  • Cancer Research

Background:

  • Proper chromosome segregation is essential for species survival and perpetuation.
  • Errors in chromosome segregation (aneuploidy) cause genetic instability and are linked to various diseases, including cancer, sterility, and birth defects.
  • Separase, an enzyme, plays a critical role in cell division by cleaving cohesin, enabling the transition from metaphase to anaphase.

Purpose of the Study:

  • To review the multifaceted roles of separase in cellular processes.
  • To explore separase's involvement in mitosis, meiosis, and centriole dynamics.
  • To discuss separase regulation, its diverse substrates, structural aspects, and its association with cancer as a potential therapeutic target.

Main Methods:

  • Literature review synthesizing existing research on separase.
  • Analysis of separase's function in cell cycle regulation.
  • Examination of separase's non-canonical roles and substrates.

Main Results:

  • Separase is crucial for accurate chromosome segregation during cell division.
  • Altered separase expression or activity is implicated in various diseases, particularly cancer, positioning it as an oncogene and therapeutic target.
  • Separase is also essential for centriole separation and duplication.
  • A proposed model suggests separase is active throughout the cell cycle, with heightened activity during metaphase, challenging previous assumptions.

Conclusions:

  • Separase is a key regulator of cell division and genetic stability.
  • Its dysregulation is linked to significant medical conditions, underscoring its importance in human health.
  • Further research into separase's mechanisms and therapeutic targeting holds promise for treating diseases like cancer.