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Microtiter-Format Assays for HBV Antigen Quantitation in Nonclinical Applications
Cally D Goddard1, Lale Bildrici-Ertekin1, Xiaohe Wang2
1Baruch S. Blumberg Institute, 3805 Old Easton Road, Doylestown, PA, 18902, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 16, 2016
Abstract:
Measurement of secreted HBV antigens in cell culture is an important endpoint in many experimental settings. Here we describe convenient and inexpensive protocols for 96-well format sandwich ELISA assays for this purpose, in which there are many options for customization of antibodies used and other parameters. These protocols can be adapted to use for animal serum samples, compound library screening, and other purposes.
