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Updated: Mar 10, 2026

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Published on: September 25, 2019

Hydrodynamic HBV Transfection Mouse Model.

Li-Ling Wu¹, Hurng-Yi Wang¹, Pei-Jer Chen²

¹Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.

Methods in Molecular Biology (Clifton, N.J.)

|December 16, 2016

Summary

This summary is machine-generated.

Hydrodynamic HBV transfection is a method for delivering macromolecules into mouse liver cells. This technique uses rapid infusion of a solution containing the HBV plasmid construct for efficient intracellular delivery.

Keywords:

Gene transfer HBV persistence Hydrodynamic injection In vivo transfection Liver tolerance

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Area of Science:

* Molecular Biology
* Virology
* Animal Models

Background:

* Hydrodynamic transfection is a key technique for intracellular delivery of macromolecules.
* This method has been established in the last decade for various applications.
* It enables the delivery of substances not normally permeable to cell membranes.

Purpose of the Study:

* To describe the fundamental principles of hydrodynamic transfection.
* To detail the application of this method in Hepatitis B Virus (HBV) mouse models.
* To provide a foundational understanding for researchers utilizing this technique.

Main Methods:

* Rapid infusion of a large volume of solution containing the HBV plasmid construct into the mouse circulation.
* Exploiting the transient permeability of liver parenchymal cells to macromolecules.
* Establishing a mouse model for studying HBV gene delivery and function.

Main Results:

* Successful intracellular delivery of macromolecules to liver cells was achieved.
* The hydrodynamic method demonstrated preferential uptake by liver parenchymal cells.
* The HBV plasmid construct was effectively delivered, enabling further study.

Conclusions:

* Hydrodynamic transfection is an effective method for in vivo gene delivery in mouse models.
* This technique facilitates the study of Hepatitis B Virus (HBV) in a controlled experimental setting.
* Understanding the principles of hydrodynamic transfection is crucial for its successful application in research.