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Updated: Mar 10, 2026

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity
Published on: March 31, 2023
Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells
Atsushi Yagishita1, Tasuku Ueno2, Hiroyasu Esumi3
1Division of Gene Regulation, Institute for Advanced Medical Research, Graduate School of Medicine, Keio University , 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582 Japan.
Abstract:
Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.
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