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Updated: Mar 10, 2026
Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection
Published on: June 23, 2023
Long non-coding RNA MVIH acts as a prognostic marker in glioma and its role in cell migration and invasion
1Department of Neurology, Weifang People's Hospital, Weifang, Shandong, China. j0901jop@163.com.
Objective:
High expression levels of lncRNA associated with microvascular invasion in HCC (lncRNA MVIH) were found to correlate with several solid tumors. However, little is known concerning the function of MVIH in glioma. The purpose of our study is to explore the role of lncRNA MVIH in clinical glioma samples and cell lines.
Patients And Methods:
The expression levels of MVIH were analyzed in glioma surgical resection tissues and cells by RT-PCR. Additionally, the associations of MVIH expression with clinicopathological features were analyzed. Survival and Cox proportional-hazards regression analyses were performed to determine the correlation between MVIH expression levels and prognosis in the patients. The cell proliferation, migration ability, invasion ability were measured successively by CKK-8 assay, transwell and wound healing assay.
Results:
We found that MVIH was significantly upregulated in glioma cell lines and tissues. Furthermore, MVIH expression was positively correlated with KPS and WHO grade. Patients with MVIH high expression tumors had a worse overall survival compared to patients with MVIH high expression tumors. Moreover, Univariate and multivariate Cox regression analysis confirmed that MVIH was an independent risk factor for glioma. Finally, in vitro, we showed that up-regulation of MVIH expression promoted human glioma cells proliferation, invasion and migration, while down-regulation of MVIH expression suppressed human glioma cells proliferation, invasion and migration.
Conclusions:
Our findings indicated that MVIH expression may serve not only as a prognostic marker but also as a potential therapeutic target in glioma.
Insights
Long non-coding RNA MVIH is highly expressed in glioma and promotes tumor progression. High MVIH expression indicates a poor prognosis and suggests MVIH as a potential therapeutic target for glioma patients.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNA associated with microvascular invasion (lncRNA MVIH) is implicated in various solid tumors.
- The specific role of MVIH in glioma remains largely unexplored.
Purpose of the Study:
- To investigate the expression and function of lncRNA MVIH in clinical glioma samples and cell lines.
- To determine the prognostic significance of MVIH in glioma.
Main Methods:
- RT-PCR was used to analyze MVIH expression in glioma tissues and cells.
- Correlation analysis was performed between MVIH expression and clinicopathological features.
- Cell proliferation, migration, and invasion assays were conducted.
- Survival and Cox regression analyses assessed the prognostic value of MVIH.
Main Results:
- MVIH was significantly upregulated in glioma tissues and cell lines.
- High MVIH expression correlated positively with KPS and WHO grade.
- MVIH upregulation promoted glioma cell proliferation, invasion, and migration.
- High MVIH expression was associated with worse overall survival and identified as an independent risk factor.
Conclusions:
- lncRNA MVIH is upregulated in glioma and promotes tumor progression.
- MVIH serves as a potential prognostic biomarker for glioma.
- MVIH represents a promising therapeutic target for glioma treatment.
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