Neuropeptide Y Y₅ receptor localization in mouse central nervous system

Neuropeptide Y (NPY) and its receptors affect blood pressure, feeding behavior, and neurogenesis. In this study, the distribution of neurons expressing NPY Y₅ receptor (Y₅) was examined in adult mouse central nervous system by immunohistochemistry. Y₅ protein localization was investigated using polyclonal anti-Y₅ antibody, which was successfully preabsorbed with Y₅ knockout brain tissues. The preabsorbed anti-Y₅ antibody did not react with Y₅ knockout brain tissues, thus meeting the "hard specificity criterion," which is the absence of staining in tissues genetically deficient for the antigen (Pradidarcheep et al., 2008). Y₅-positive neurons were found in most brain areas. Most Y₅ immunoreactivities were observed as dot-like structures adjacent to the plasma membrane, as expected for a cell membrane receptor. In situ hybridization showed that the Y₅ mRNA expression was correlated with the Y₅ protein level in each case and that it was probably controlled by the transcriptional regulation of the Y₅ gene. In the nuclei where Y₅ was expressed, Y₅ immunoreactivities were found mainly in the somatic and dendritic areas. The distribution patterns of the Y₅-positive cells that were broader than previously expected suggest important biological activities of the Y₅ in many brain areas.