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Inhibition of Adhesion Molecule Gene Expression and Cell Adhesion by the Metabolic Regulator PGC-1α

Neri Minsky1, Robert G Roeder1

  • 1Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York, United States of America.

Plos One
|December 17, 2016
PubMed

Insights

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) inhibits cell adhesion molecule expression. This study reveals PGC-1α as a key molecular link between metabolic regulation and cell adhesion processes.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Metabolic Regulation

Background:

  • Cell adhesion is crucial for cell shape and function in physiological and pathological states.
  • The interplay between cellular metabolism and cell adhesion is not fully understood.
  • PGC-1α is a key regulator of metabolic gene expression.

Purpose of the Study:

  • To investigate the role of PGC-1α in regulating cell adhesion.
  • To elucidate the molecular mechanisms linking metabolism and cell adhesion.
  • To identify PGC-1α as a potential mediator between these cellular networks.

Main Methods:

  • Utilized cell lines, primary cells, and mouse models.
  • Assessed gene expression of cell adhesion molecules.
  • Performed mRNA stability and intronic RNA expression analyses.
  • Investigated the role of PGC-1α's L2/L3 motifs.
  • Examined the effect of PGC-1α on cell adhesion to extracellular matrix proteins.

Main Results:

  • PGC-1α significantly down-regulates the expression of various cell adhesion molecules.
  • Evidence suggests PGC-1α acts transcriptionally to inhibit cell adhesion genes.
  • Specific PGC-1α motifs (L2/L3) are partially required for this inhibitory effect.
  • PGC-1α modulates the adhesion of primary fibroblasts and hepatic stellate cells.

Conclusions:

  • PGC-1α acts as an inhibitor of cell adhesion gene expression.
  • A novel cross-talk exists between metabolic regulation and cell adhesion pathways.
  • PGC-1α serves as a molecular link connecting cellular metabolism and cell adhesion.

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