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Detection of Babesia microti parasites by highly sensitive 18S rRNA reverse transcription PCR
Amelia E Hanron1, Zachary P Billman2, Annette M Seilie1
1Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.
Abstract:
Babesia are increasingly appreciated as a cause of transfusion-transmitted infection. Sensitive methods are needed to screen blood products. We report herein that B. microti 18S rRNA is over 1,000-fold more abundant than its coding genes, making reverse transcription PCR (RT-PCR) much more sensitive than PCR. Babesia 18S rRNA may be useful for screening the blood supply.
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