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Related Experiment Video

Updated: Mar 9, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

Three-color crystal digital PCR.

J Madic¹, A Zocevic¹, V Senlis²

¹Stilla Technologies, 1 Mail du Professeur Georges Mathé, 94800 Villejuif, France.

Biomolecular Detection and Quantification

|December 20, 2016

Summary

This summary is machine-generated.

Crystal Digital PCR™ offers precise nucleic acid quantification and rare event detection using a novel chip-based, three-color multiplexing system. This technology enhances molecular analysis with a user-friendly workflow and advanced data analysis for complex samples.

Keywords:

Crystal Digital PCR EGFR Multicolor PCR Multiplex digital PCR NSCLC Naica

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Area of Science:

Molecular Biology
Biotechnology
Genomics

Background:

Digital PCR (dPCR) provides high precision for nucleic acid quantification.
Detecting rare molecular events in complex samples remains a challenge.

Purpose of the Study:

Introduce Crystal Digital PCR™, a novel technology for enhanced molecular analysis.
Demonstrate the capabilities of a three-color multiplexing system in dPCR.

Main Methods:

Utilizes a single chip for partitioning samples into 2D droplet arrays.
Employs thermal cycling followed by three-color fluorescence scanning.
Develops a new data analysis workflow for multiplexed dPCR.

Main Results:

Achieved three-color multiplexing for simultaneous detection of multiple targets.
Presented a fast and user-friendly workflow for digital PCR.
Demonstrated proof-of-concept with a quadriplex assay for EGFR mutations (L858R, L861Q, T790M).

Conclusions:

Crystal Digital PCR™ offers a powerful platform for precise nucleic acid quantification.
The developed workflow and data analysis address challenges in multiplexed dPCR.
This technology advances the detection of rare mutations and complex molecular events.