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Updated: Mar 9, 2026
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
Suppressive effect of microRNA-143 in retinoblastoma
Li-Lun Wang1, Hai-Feng Hu2, Yan-Qin Feng1
1Department of Ophthalmology, Affiliated Hospital of Yan'an University, Yan'an 716000, Shaanxi Province, China.
Aim:
To investigate microRNA-143 expression and effect on suppression of retinoblastoma (RB) cells.
Methods:
The expression of microRNA-143 was investigated and compared in normal human retina tissue samples and in RB cell lines of Y79 and Weri1. The microRNA-143 mimics were transfected into the RB cell lines separately, and its effect on RB cell lines was detected using reverse-transcription quantitative polymerase chain reaction and Western blotting methods.
Results:
The microRNA-143 expression was significantly suppressed in RB cell lines. Overexpression of microRNA-143 significantly lowered cell viability and invasion of the RB cell lines, and increased the number of apoptotic cells. Meanwhile, the Bax expression was up-regulated and much higher in the microRNA-143 mimics transfected group than that in the negative control and the microRNA-143 inhibitor groups.
Conclusion:
MicroRNA-143 exhibits suppressive effects in RB. The current study provides the perspective of a potential therapeutic treatment for RB.
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