Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptional divergence of the zebrafish <i>sox17</i> lineage begins during gastrulation.

bioRxiv : the preprint server for biology·2026
Same author

Lactation Interrupted: PFAS Impact on Capacity to Breastfeed Ignored.

Current environmental health reports·2026
Same author

Long-range mutual activation establishes Rho and Rac polarity during cell migration.

Nature cell biology·2026
Same author

A conserved antioxidant defense at the endoplasmic reticulum membrane.

Cell reports·2026
Same author

Met regulates endoderm migration in zebrafish.

bioRxiv : the preprint server for biology·2026
Same author

Context-dependent variability of HIF heterodimers influences interactions with macromolecular and small molecule partners.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Mar 9, 2026

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System
05:28

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

Published on: August 19, 2021

3.5K

TAEL: a zebrafish-optimized optogenetic gene expression system with fine spatial and temporal control.

Anna Reade1, Laura B Motta-Mena2, Kevin H Gardner2,3,4

  • 1CVRI & Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.

Development (Cambridge, England)
|December 21, 2016
PubMed
Summary

Researchers developed a new optogenetic gene expression system, TAEL (TA4-EL222), for zebrafish. This system offers precise spatial and temporal control of gene expression with minimal toxicity, advancing developmental biology research.

Keywords:
CRISPRCas9EndodermGene expressionNodalOptogeneticsZebrafish

More Related Videos

Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions
07:18

Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions

Published on: October 27, 2023

3.4K
Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato
07:25

Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato

Published on: January 31, 2013

15.3K

Related Experiment Videos

Last Updated: Mar 9, 2026

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System
05:28

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

Published on: August 19, 2021

3.5K
Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions
07:18

Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions

Published on: October 27, 2023

3.4K
Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato
07:25

Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato

Published on: January 31, 2013

15.3K

Area of Science:

  • Developmental Biology
  • Genetics
  • Optogenetics

Background:

  • Existing optogenetic systems exhibit toxicity in zebrafish, limiting their application.
  • Robust spatial and temporal regulation of gene expression is crucial for studying developmental processes.

Purpose of the Study:

  • To engineer a novel, low-toxicity optogenetic gene expression system for zebrafish.
  • To achieve precise spatial and temporal control over gene induction in vivo.
  • To demonstrate the utility of the system in studying developmental signaling and gene editing.

Main Methods:

  • Re-engineering the blue-light-activated EL222 system to create the TAEL (TA4-EL222) system.
  • Validating strategies for spatially restricted illumination to control gene induction.
  • Utilizing TAEL for targeted sox32 induction to generate ectopic endodermal cells.
  • Applying TAEL to investigate Nodal signaling roles during embryonic development.
  • Combining TAEL with CRISPR/Cas9 for inducible gene editing.

Main Results:

  • The TAEL system demonstrates minimal toxicity in zebrafish.
  • Achieved robust induction with fine spatial precision and rapid kinetics.
  • Successfully induced ectopic endodermal cells via targeted sox32 expression.
  • Resolved distinct roles of Nodal signaling at different embryonic stages.
  • Demonstrated inducible gene editing using TAEL and CRISPR/Cas9.

Conclusions:

  • The TAEL system provides a powerful, versatile tool for optogenetic control in zebrafish.
  • This toolkit enables precise manipulation of gene expression for studying developmental biology and gene editing.
  • TAEL is expected to be a valuable resource for the zebrafish research community.