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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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In-vitro Mutagenesis01:16

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Related Experiment Video

Updated: Mar 9, 2026

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
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Efficient CRISPR/Cas9-based gene knockout in watermelon.

Shouwei Tian1, Linjian Jiang2, Qiang Gao3

  • 1National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, 100097, China.

Plant Cell Reports
|December 21, 2016
PubMed
Summary
This summary is machine-generated.

The CRISPR/Cas9 system precisely edits watermelon genomes, creating knockout mutations in T0 plants. This powerful genome editing tool efficiently reveals gene function and generates valuable crop mutations.

Keywords:
CRISPR/Cas9Genome editingPDSWatermelon

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Area of Science:

  • Plant genetics
  • Molecular biology
  • Agricultural science

Background:

  • The clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) system enables targeted genome editing.
  • Previous studies have demonstrated CRISPR/Cas9's efficacy in various plant species for mutagenesis.

Purpose of the Study:

  • To investigate the applicability and efficiency of the CRISPR/Cas9 system for genome editing in watermelon (Citrullus lanatus).
  • To generate targeted mutations in the ClPDS gene and assess their phenotypic effects in watermelon.

Main Methods:

  • Utilized the CRISPR/Cas9 system to target the ClPDS gene in watermelon protoplast cells.
  • Generated transgenic watermelon plants and analyzed genomic DNA for mutations.
  • Phenotypically evaluated transgenic plants for albino traits associated with ClPDS gene disruption.
  • Bioinformatically examined potential off-target mutation sites.

Main Results:

  • CRISPR/Cas9 successfully induced insertions or deletions at the target site in watermelon protoplasts.
  • All generated transgenic watermelon plants exhibited ClPDS mutations, resulting in albino phenotypes.
  • Achieved 100% genome editing efficiency in the T0 generation of transgenic watermelon lines.
  • Analysis indicated a low likelihood of off-target mutations in the examined regions.

Conclusions:

  • The CRISPR/Cas9 system is a highly effective tool for precise genome editing in watermelon.
  • This technology facilitates gene function studies and the development of desirable mutations in watermelon.
  • Demonstrated the potential for rapid generation of knockout mutations in crop plants using CRISPR/Cas9.