Characterization and complete genome sequence of a panicovirus from Bermuda grass by high-throughput sequencing

Area of Science:

• Plant Virology
• Molecular Biology
• Genomics

Background:

• Bermuda grass is a globally significant turf and forage grass.
• Emerging viral diseases can impact plant health and agricultural productivity.

Purpose of the Study:

• To identify and characterize a novel virus found in Bermuda grass.
• To determine the taxonomic classification of the newly discovered virus.

Main Methods:

• Transmission electron microscopy (TEM) for visualizing virus particles.
• High-throughput sequencing (HTS) for initial genome identification.
• Sanger sequencing for genome confirmation and completion.

Main Results:

• Spherical virus particles (28-30 nm) were observed in Bermuda grass samples.
• A nearly full genome sequence of a panicovirus was identified via HTS.
• The complete genome sequence (4404 nt) of Bermuda grass latent virus (BGLV) was determined.

Conclusions:

• Bermuda grass latent virus (BGLV) is a new species within the genus Panicovirus, family Tombusviridae.
• Phylogenetic analysis and sequence comparisons confirm BGLV's distinctness from known viruses.
• This discovery expands our understanding of panicoviruses affecting important grass species.