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Updated: Mar 9, 2026
Basophil Activation Test for Allergy Diagnosis
Published on: May 31, 2021
Exploratory analysis of CD63 and CD203c expression in basophils from hazelnut sensitized and allergic individuals
Bianca Lötzsch1, Sabine Dölle1, Stefan Vieths2
1Department of Dermatology and Allergology, Allergy-Center-Charité, Charité - Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany.
Background:
Sensitization to hazelnut (HN) is frequent and requires clarification to determine whether this sensitization is clinically relevant. The aim of this study was to investigate basophil activation profiles in HN-sensitized and allergic subjects.
Methods:
Basophil activation was determined by flow cytometric analyses of CD63 and CD203c expression using several HN allergen concentrations. Depending on their clinical reaction pattern, an oral allergy symptom group (OAS, n = 20), a systemic reaction group (n = 12) and a sensitized group without clinical symptoms (n = 20) were identified. Additionally, 10 non-allergic and non-sensitized individuals served as controls.
Results:
CD63 and CD203c expression differed between allergic (OAS and systemic group) and sensitized subjects. The HN concentration required to activate 30% of CD203c+ basophils [effective concentration (EC)30] was significantly higher in sensitized versus the allergic group (p = 0.0089). This was more pronounced when the basophil allergen threshold sensitivity (CD-sens) was calculated (CD63: p = 0.018; CD203c: p = 0.009).
Conclusion:
Our data indicate that the basophil activation test may provide information to better distinguish between sensitized and allergic subjects if several allergen concentrations are considered. CD203c expression displayed a better discrimination compared to CD63; therefore, its diagnostic value might be superior compared with CD63.
