Micro-CT and optical microscopy imagistic investigations of root canal morphology

Protecting the root's internal morphology is the first key toward the success of the endodontic treatment. Due to the vast diversity of endodontic space, it is difficult to visualize and to establish the shape and limits of the root canal, especially the morphology of apical area and lateral root canals. Optical microscopy is a classical imagistic investigation method, widely used along classical methods like radiographs that also offer limited information about root morphology and extension of decay. Micro-computed tomography (micro-CT), a modern imagistic investigation method can provide detailed three-dimensional reconstructions of root canal. Micro-CT is a non-invasive method that has the possibility to offer cross-sectional and axial images of the endodontic space. The success of root canal treatment is based on cleaning and shaping. Beyond these two procedures, sealing the endodontic space by respecting its limits is another prerequisite for long-term success of endodontic therapy. Micro-CT can perform three-dimensional reconstruction of the root canal, root canal filling and can provide accurate images of the endodontic space. The assessment of root morphology can be obtained through imagistic invasive optical microscopy and already mentioned non-invasive methods (micro-CT). The aim of this study is to illustrate and analyze the endodontic space, according to its diversity by using micro-CT, a non-invasive imagistic investigation method an also optical microscopy. The two techniques can also provide the extension of carries or demineralized substance on different levels of the root.