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Related Experiment Video

Updated: Mar 9, 2026

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Multiplexed Single-Cell Imaging: Past, Present, and Future.

¹Laboratory of Systems Pharmacology, HMS LINCS Center , Harvard Medical School Boston, Boston, Massachusetts.

Assay and Drug Development Technologies

|December 23, 2016

Summary

This summary is machine-generated.

Jia-Ren Lin developed cyclic immunofluorescence (CycIF), a novel single-cell imaging technique for detecting multiple antigens. This innovative method shows promise across various stages of drug development.

Keywords:

cyclic immunofluorescence drug discovery multiplexed imaging

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Last Updated: Mar 9, 2026

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

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Area of Science:

Biomolecular Imaging
Systems Pharmacology
Single-Cell Analysis

Background:

Traditional methods struggle with simultaneous detection of numerous antigens in single cells.
Advancements in imaging are crucial for understanding complex biological systems and disease mechanisms.

Purpose of the Study:

To introduce and summarize the cyclic immunofluorescence (CycIF) method.
To highlight the potential applications of CycIF in drug development.
To outline current research and future directions for CycIF.

Main Methods:

Development of cyclic immunofluorescence (CycIF) for multiplexed antigen detection.
Application of CycIF in single-cell imaging studies.
Utilizing CycIF for drug discovery, preclinical, and clinical research.

Main Results:

Jia-Ren Lin received the best poster award for his work on CycIF.
CycIF enables simultaneous detection of multiple antigens at the single-cell level.
The method is adaptable for diverse applications in biomedical research.

Conclusions:

Cyclic immunofluorescence (CycIF) is a powerful new tool for high-plex single-cell imaging.
CycIF has broad applicability in various phases of drug development.
Further development of CycIF will enhance its utility in biomedical research.