[COMPARATIVE ANALYSIS OF ANTIBACTERIAL EFFECT OF PHYTOANTIBIOTICS AND ANTIBIOTICS]

Area of Science:

• Pharmacology
• Microbiology
• Natural Products Chemistry

Background:

• Nosocomial infections pose a significant threat, often driven by antibiotic-resistant bacteria.
• Conventional antibiotics face challenges due to rising antimicrobial resistance.

Purpose of the Study:

• To evaluate the antibacterial efficacy of specific phyto-medicines against key nosocomial pathogens.
• To compare the antimicrobial activity of these natural compounds with established antibiotics.

Main Methods:

• Antibiotic resistance profiling of four bacterial strains (Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Acinetobacter spp.) using the Kirby-Bauer diffusion method.
• Determination of antibacterial activity of Sanguinarine, Chelerythrine, Berberine hydrochloride, and essential oils/aromatic waters (Eucalyptus, Salvia, Lavanda) via a modified Raits serological dilution method.

Main Results:

• Phyto-medicines showed significant antibacterial activity, surpassing that of tested antibiotics.
• No increase in antibiotic-resistant bacteria was observed in undiluted and 1:1 dilutions of phyto-medicines.
• Limited bacterial growth was noted at 1:5 dilutions, with intensive growth in further dilutions.

Conclusions:

• The studied phyto-medicines exhibit potent antibacterial properties against nosocomial infection-causing bacteria.
• These natural compounds represent a promising alternative or adjunct to conventional antibiotics in combating resistant infections.