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Updated: Mar 9, 2026
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Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models
Gloria Allocca1, Anjali P Kusumbe2, Saravana K Ramasamy3
1Department of Oncology and Metabolism, The Mellanby Centre for Bone Research, The University of Sheffield , Sheffield, UK.
Abstract:
Confocal and two-photon microscopy has been widely used in bone research to not only produce high quality, three-dimensional images but also to provide valuable structural and quantitative information. In this article, we describe step-by-step protocols for confocal and two-photon microscopy to investigate earlier cellular events during colonisation of cancer cells in bone using xenograft mouse models. This includes confocal/two-photon microscopy imaging of paraformaldehyde fixed thick bone sections and frozen bone samples.
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