Role of BK_Ca in Stretch-Induced Relaxation of Colonic Smooth Muscle

Stretch-induced relaxation has not been clearly identified in gastrointestinal tract. The present study is to explore the role of large conductance calcium-activated potassium channels (BK_Ca) in stretch-induced relaxation of colon. The expression and currents of BK_Ca were detected and the basal muscle tone and contraction amplitude of colonic smooth muscle strips were measured. The expression of BK_Ca in colon is higher than other GI segments (P < 0.05). The density of BK_Ca currents was very high in colonic smooth muscle cells (SMCs). BK_Ca in rat colonic SMCs were sensitive to stretch. The relaxation response of colonic SM strips to stretch was attenuated by charybdotoxin (ChTX), a nonspecific BK_Ca blocker (P < 0.05). After blocking enteric nervous activities by tetrodotoxin (TTX), the stretch-induced relaxation did not change (P > 0.05). Still, ChTX and iberiotoxin (IbTX, a specific BK_Ca blocker) attenuated the relaxation of the colonic muscle strips enduring stretch (P < 0.05). These results suggest stretch-activation of BK_Ca in SMCs was involved in the stretch-induced relaxation of colon. Our study highlights the role of mechanosensitive ion channels in SMCs in colon motility regulation and their physiological and pathophysiological significance is worth further study.