Optimized procedures for manganese-52: Production, separation and radiolabeling

Area of Science:

• Nuclear Chemistry
• Radiopharmaceutical Chemistry

Background:

• Manganese-52 (⁵²Mn) is a valuable radioisotope for preclinical imaging.
• Efficient production and purification methods are crucial for its application.

Purpose of the Study:

• To develop an improved method for the production and purification of ⁵²Mn.
• To achieve high yields and purity of ⁵²Mn for radiolabeling.

Main Methods:

• Irradiation of chromium targets with 16MeV protons.
• Solid-phase anion exchange separation using ethanol-HCl mixtures.
• AG 1-X8 column chromatography for impurity removal.
• Rapid radiolabeling of DOTA with ⁵²Mn.

Main Results:

• Achieved average yields of 6.2±0.8MBq/µAh for ⁵²Mn.
• Recovered 94.3±1.7% of ⁵²Mn with a chromium reduction factor of 2.2±0.4×10⁵.
• Obtained >99% radiochemical yield for ⁵²Mn-DOTA within 1 minute.
• Demonstrated stability of ⁵²Mn-DOTA in bovine serum for over 2 days.

Conclusions:

• The developed separation and purification methodology significantly enhances ⁵²Mn purity.
• This improved process facilitates the use of ⁵²Mn in basic science and preclinical studies.