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Related Experiment Videos

Lysosomal enzyme abnormalities in keratoconus.

S Sawaguchi1, B Y Yue, J Sugar

  • 1Department of Opthalmology, Lions of Illinois Eye Research Institute, University of Illinois, College of Medicine, Chicago 60612.

Archives of Ophthalmology (Chicago, Ill. : 1960)
|October 1, 1989
PubMed
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Keratoconus corneas show elevated lysosomal enzyme activity, particularly in the epithelium. This suggests abnormal tissue degradation processes may contribute to this corneal disease.

Area of Science:

  • Ophthalmology
  • Biochemistry
  • Cell Biology

Background:

  • Keratoconus is a progressive thinning of the cornea.
  • The role of lysosomal enzymes in corneal diseases is not fully understood.
  • Previous research has speculated on the involvement of the corneal epithelium in keratoconus.

Purpose of the Study:

  • To investigate lysosomal enzyme activities in keratoconus corneas.
  • To compare enzyme levels in keratoconus, normal, scarred, and Fuchs' dystrophy corneas.
  • To explore the potential involvement of corneal epithelium in keratoconus pathogenesis.

Main Methods:

  • Histochemical staining for acid phosphatase, acid esterase, and acid lipase.
  • Analysis of enzyme activity using an image-processing system.

Related Experiment Videos

  • Comparison of enzyme levels in corneal tissues from keratoconus patients and controls.

    • Main Results:

    • Significantly higher levels of acid phosphatase, acid esterase, and acid lipase were found in the epithelium of keratoconus corneas compared to normal corneas.
    • Elevated enzyme activity was most pronounced in the basal epithelium.
    • No such abnormalities were observed in scarred corneas or corneas with Fuchs' corneal dystrophy.

    Conclusions:

    • The findings support the hypothesis that abnormal tissue degradation processes occur in keratoconus.
    • The corneal epithelium appears to be involved in keratoconus.
    • Lysosomal enzyme activity may serve as a biomarker for keratoconus progression or severity.