Related Experiment Video

Updated: Mar 9, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.1K

The pre-existing population of 5S rRNA effects p53 stabilization during ribosome biogenesis inhibition

Carmine Onofrillo1, Alice Galbiati1, Lorenzo Montanaro1

  • 1Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, S. Orsola Hospital, 40138, Bologna, Italy.

Oncotarget
|December 30, 2016
PubMed

Insights

The central MDM2 inhibitory complex, the 5S RNP, controls p53 stabilization. MDM2 inhibition relies on a pre-existing 5S rRNA population, clarifying 5S RNP assembly dynamics.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Cancer Research

Background:

  • The RPL5/RPL11/5S rRNA (5S RNP) complex is a key inhibitor of MDM2.
  • This complex stabilizes p53 protein levels, particularly during ribosome biogenesis stress.
  • The precise dynamics of 5S RNP assembly remain incompletely understood.

Purpose of the Study:

  • To investigate the dynamic assembly of the 5S RNP.
  • To elucidate the role of pre-existing 5S rRNA in MDM2 inhibition.
  • To further characterize the regulation of p53 stabilization during ribosome biogenesis inhibition.

Main Methods:

  • Analysis of pre-ribosomal complex formation.
  • Investigating the kinetics of 5S RNP assembly.
  • Assessing MDM2 inhibition in relation to 5S rRNA availability.

Main Results:

  • MDM2 inhibition is contingent upon the presence of a pre-existing 5S rRNA pool.
  • The study provides new insights into the assembly dynamics of the 5S RNP.
  • This finding clarifies a critical step in the p53-MDM2 regulatory pathway.

Conclusions:

  • A pre-existing 5S rRNA population is essential for effective MDM2 inhibition.
  • Understanding 5S RNP assembly dynamics is crucial for comprehending p53 regulation.
  • These findings contribute to the molecular understanding of ribosome biogenesis stress responses.
Keywords:
5S rRNAMDM2p53ribosomal proteinsribosome biogenesis inhibition

More Related Videos

Purification of Ubiquitinated p53 Proteins from Mammalian Cells10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.8K
Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

291

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.7K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.3K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
431
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
36.0K