Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

3.2K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
3.2K
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

4.0K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
4.0K
Pigmentation01:19

Pigmentation

4.8K
The color of the skin is influenced by a number of pigments, including melanin, carotene, and hemoglobin. Recall that melanin is produced by cells called melanocytes, which are found scattered throughout the stratum basale of the epidermis. The melanin is transferred to the keratinocytes via melanosomes.
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
4.8K
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

3.4K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
3.4K
Changes in Skin Color: Clinical Perspectives01:14

Changes in Skin Color: Clinical Perspectives

4.0K
The first thing a clinician sees is the skin, so the examination of the skin should be part of any thorough physical examination. Most skin disorders are relatively benign, but a few, including melanomas, can be fatal if untreated. A couple of the more noticeable disorders, albinism and vitiligo, affect the appearance of the skin and its accessory organs.
Albinism
Albinism is a genetic disorder that affects (completely or partially) the coloring of skin, hair, and eyes. The defect is primarily...
4.0K
Role of Skin in Vitamin D Synthesis01:23

Role of Skin in Vitamin D Synthesis

8.5K
The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...
8.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tracing PFAS Transfer from Mother to the Fetoplacental Unit: Insights from Trimester-Specific Maternal Serum Profiles.

bioRxiv : the preprint server for biology·2026
Same author

DNA methylation signatures of inflammation in youth living with perinatally-acquired HIV.

Clinical epigenetics·2026
Same author

Protecting the developing brains of children from Plastics-Derived chemicals and microplastic particles.

Environment international·2026
Same author

Detailed Protocol for Expansion of Large Tissue Samples for Expansion Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Evaluation of Epithelial Integrity in Human Precision-Cut Kidney Slices.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same author

Oculomics meets exposomics: a roadmap for applying multi-modal ocular biomarkers in precision environmental health research.

Exposome·2025

Related Experiment Video

Updated: Mar 9, 2026

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair
07:32

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Published on: February 17, 2021

8.9K

Using neonatal skin to study the developmental programming of aging.

Leryn J Reynolds1, Brett J Dickens1, Benjamin B Green2

  • 1Department of Pharmacology and Nutritional Sciences, University of Kentucky, Lexington, KY 40536, USA.

Experimental Gerontology
|December 31, 2016
PubMed
Summary
This summary is machine-generated.

Human neonatal foreskin offers a new way to study how early-life exposures impact obesity and diabetes risk. This research shows foreskin cells can model key processes like fat development and insulin response.

Keywords:
AdipogenesisDOHaDEpigeneticsInsulin sensitivityObesityPregnancy

More Related Videos

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
09:16

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Published on: December 14, 2015

12.0K
SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
07:39

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

Published on: June 28, 2019

25.4K

Related Experiment Videos

Last Updated: Mar 9, 2026

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair
07:32

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Published on: February 17, 2021

8.9K
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
09:16

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Published on: December 14, 2015

12.0K
SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
07:39

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

Published on: June 28, 2019

25.4K

Area of Science:

  • Developmental biology
  • Metabolic disease research
  • Human neonate studies

Background:

  • Maternal exposures during pregnancy can influence offspring's risk for chronic diseases.
  • Obesity and type 2 diabetes are significant age-associated diseases.
  • Understanding developmental programming is crucial for preventing these conditions.

Purpose of the Study:

  • To introduce human neonatal foreskin as a novel model for studying developmental programming.
  • To investigate adipogenesis and insulin receptor signaling in neonatal foreskin.
  • To link neonatal development to long-term risks of obesity and diabetes.

Main Methods:

  • Collected neonatal foreskin tissue post-circumcision.
  • Isolated primary dermal fibroblasts from the foreskin.
  • Performed in vitro adipocyte differentiation and insulin stimulation assays.

Main Results:

  • Human neonatal foreskin fibroblasts successfully undergo lipid uptake when stimulated for differentiation.
  • Foreskin fibroblasts exhibit functional insulin receptor signaling upon insulin stimulation.
  • These findings validate the foreskin model for studying metabolic processes.

Conclusions:

  • Neonatal foreskin is a viable and novel human model for studying developmental programming.
  • This model can be used to investigate the impact of early-life exposures on adipogenesis and insulin signaling.
  • Foreskin research may provide insights into preventing neonatal obesity and diabetes.