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Molecular diagnosis of bacterial vaginosis: Does adjustment for total bacterial load or human cellular content
E L Plummer1, S M Garland2, C S Bradshaw3
1Department of Molecular Microbiology, Murdoch Childrens Research Institute, Melbourne, Australia; Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia.
Abstract:
We investigated the utility of quantitative PCR assays for diagnosis of bacterial vaginosis and found that while the best model utilized bacterial copy number adjusted for total bacterial load (sensitivity=98%, specificity=93%, AUC=0.95[95%CI=0.93,0.97]), adjusting for total bacterial or human cell load did not consistently increase the diagnostic performance of the assays.
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