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Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
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Tempol effect on epithelial-mesenchymal transition induced by hyperglycemia
Mohammad Jafari1, Farahnaz Dadras2, Hamid Reza Ghadimipour1
1Pathology ward, Hamadan University of Medical Sciences, Hamadan, Iran.
Journal of Nephropathology
|January 3, 2017
Summary
The antioxidant tempol partially modified epithelial-mesenchymal transition (EMT) in diabetic nephropathy (DN) rat kidneys, but did not significantly reverse the pathological changes associated with this chronic kidney disease mechanism.
Area of Science:
- Nephrology
- Pathophysiology
- Pharmacology
Background:
- Epithelial-mesenchymal transition (EMT) is a key mechanism in chronic kidney disease progression.
- Oxidative stress contributes to kidney damage in diabetic nephropathy (DN).
Purpose of the Study:
- To investigate the potential of tempol, an antioxidant, to ameliorate EMT in a rat model of DN.
- To assess the impact of tempol on kidney pathology and EMT markers in diabetic rats.
Main Methods:
- A DN model was induced in male rats, divided into control, diabetic, tempol-treated, and diabetic+tempol groups.
- Rats received daily gavage of tempol (100 mg/kg/day) for 28 days.
- Kidney tissues were analyzed using immunohistochemical staining for EMT markers (E-cadherin, α SMA).
Main Results:
- Diabetic rats exhibited increased kidney weight and urinary albumin excretion, indicative of kidney damage.
- Significant decreases in E-cadherin and increases in α SMA staining were observed in renal tubular cells of diabetic rats.
- Tempol treatment partially modified these EMT indices in diabetic rats.
Conclusions:
- Tempol demonstrated a partial modifying effect on EMT in diabetic nephropathy.
- The antioxidant did not significantly reverse the EMT process induced by DN in this study.
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