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The putative serine protease inhibitor (serpin) genes encoded on Echinococcus multilocularis genome and their

Mizuki Sasaki1, Yasuhito Sako1

  • 1Department of Parasitology, Asahikawa Medical University, 2-1-1-1, Midorigaoka-Higashi, Asahikawa, Hokkaido, 078-8510, Japan.

Veterinary Parasitology
|January 4, 2017
PubMed

Insights

Researchers identified two new serpin genes in Echinococcus multilocularis, named serpin2Emu and serpin3Emu. These, along with serpinEmu, are present in parasite tissues and excretory-secretory fractions.

Area of Science:

  • Parasitology
  • Molecular Biology
  • Biochemistry

Background:

  • Echinococcus multilocularis is a parasitic cestode causing cystic echinococcosis.
  • Serine protease inhibitors (serpins) play crucial roles in regulating protease activity.
  • The serpinEmu gene was previously identified in E. multilocularis.

Purpose of the Study:

  • To identify and characterize novel serpin genes in Echinococcus multilocularis.
  • To investigate the localization of identified serpin proteins within the parasite.

Main Methods:

  • Gene identification and sequencing.
  • Western blot analysis using specific polyclonal antibodies.
  • Immunohistochemical staining of parasite tissues.

Main Results:

  • Two novel putative serpin genes, designated serpin2Emu and serpin3Emu, were identified in E. multilocularis.
  • Western blot confirmed the presence of serpinEmu, serpin2Emu, and serpin3Emu proteins in both intracellular and excretory-secretory (ES) fractions.
  • Immunohistochemical analysis revealed that all three serpin proteins are localized in the germinal layer of E. multilocularis metacestodes.

Conclusions:

  • Echinococcus multilocularis possesses at least three distinct serpin genes.
  • The identified serpin proteins are secreted and also located within the parasite's germinal layer.
  • These findings contribute to understanding the molecular mechanisms and immune evasion strategies of E. multilocularis.
Keywords:
Alveolar echinococcosisEchinococcus multilocularisMetacestodeSerine protease inhibitor

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