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Intrinsic blinking of red fluorescent proteins for super-resolution microscopy
Natalia V Klementieva1, Anton I Pavlikov1, Alexander A Moiseev2
1Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
Abstract:
Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.
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