Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Asymmetric Doping of a Polyelectrolyte Network Into a Tough Slide-Ring Hydrogel Membrane to Enhance Sustainable Osmotic Energy Harvesting.

Small science·2026

Same author

Viral capsid delivery of cGAMP enhances STING-dependent antitumor immune response.

bioRxiv : the preprint server for biology·2026

Same author

Clinical evaluation of photochromic nanoparticle tattoo ink: safety, tolerability, and performance of rewritable intradermal implants.

Journal of nanobiotechnology·2026

Same author

Granular Extracellular Matrix (gECM) Hydrogels Enable Distinct Composition and Mechanics Across Tissue Types for Translation.

bioRxiv : the preprint server for biology·2026

Same author

Reliability-Aware Deep Learning Framework for Chemical Genotoxicity Prediction with Uncertainty Quantification.

Journal of chemical information and modeling·2026

Same author

Synergistic Dual Slip-Link Toughening of a Water-Rich Double Network Hydrogel Combining Slide-Ring and Highly Entangled Networks.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Search research articles

Related Experiment Video

Updated: Mar 9, 2026

Hyperpolarized Xenon for NMR and MRI Applications

Hyperpolarized Xenon for NMR and MRI Applications

Published on: September 6, 2012

Rotaxane probes for protease detection by ¹²⁹Xe hyperCEST NMR.

Clancy C Slack¹, Joel A Finbloom², Keunhong Jeong¹

¹Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA. mbfrancis@berkeley.edu and Materials Sciences Division, Lawrence Berkeley National Laboratories, Berkeley, CA 94720, USA.

Chemical Communications (Cambridge, England)

|January 4, 2017

Summary

We developed a novel rotaxane molecule for detecting matrix metalloprotease 2 (MMP-2), an important cancer marker. This new method uses ¹²⁹Xe hyperCEST NMR to enable protease-responsive imaging in cancer diagnostics.

More Related Videos

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

Published on: September 3, 2013

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

Related Experiment Videos

Last Updated: Mar 9, 2026

Hyperpolarized Xenon for NMR and MRI Applications

Hyperpolarized Xenon for NMR and MRI Applications

Published on: September 6, 2012

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

Published on: September 3, 2013

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

Area of Science:

Supramolecular Chemistry
Chemical Biology
Biomedical Imaging

Background:

Matrix metalloproteinase 2 (MMP-2) is frequently overexpressed in cancerous tissues, making it a significant biomarker for cancer detection.
Current methods for MMP-2 detection can be limited in sensitivity and specificity.
Developing novel molecular tools for sensitive and specific MMP-2 detection is crucial for advancing cancer diagnostics.

Purpose of the Study:

To develop a novel supramolecular system for the sensitive and specific detection of matrix metalloproteinase 2 (MMP-2) activity.
To utilize ¹²⁹Xe hyperCEST NMR as a detection platform for protease activity.
To create a protease-responsive molecular probe for potential cancer imaging applications.

Main Methods:

Synthesis of a cyclobis(para-phenylene-22-crown-6) (CB6) based rotaxane incorporating a peptide substrate specific for MMP-2.
Utilizing CB6-promoted click chemistry for the efficient synthesis of the rotaxane.
Employing ¹²⁹Xe hyperCEST NMR spectroscopy to monitor the interaction of ¹²⁹Xe with the CB6 cage upon MMP-2 cleavage.

Main Results:

Successful synthesis of the CB6 rotaxane with an embedded MMP-2 recognition sequence.
Demonstration that MMP-2 enzymatic activity cleaves the rotaxane, releasing the CB6 cage.
Observation of protease-responsive activation of ¹²⁹Xe@CB6 interactions, indicating successful detection of MMP-2 activity.

Conclusions:

The developed CB6 rotaxane serves as an effective molecular probe for MMP-2 activity.
¹²⁹Xe hyperCEST NMR in conjunction with this rotaxane offers a sensitive method for detecting protease activity.
This approach holds promise for the development of new diagnostic tools for cancer detection based on MMP-2 levels.