Correlative Super-Resolution Microscopy: New Dimensions and New Opportunities
Meghan Hauser1, Michal Wojcik1, Doory Kim1
1Department of Chemistry, University of California , Berkeley, California 94720, United States.
Chemical Reviews
|January 4, 2017
Summary
Super-resolution microscopy (SRM) enhances light microscopy resolution to 10 nm. Correlative SRM integrates multiple techniques, overcoming limitations and enabling new insights in biology and materials science.
Area of Science:
- Microscopy
- Biophysics
- Materials Science
Background:
- Correlative microscopy combines techniques to leverage individual strengths.
- Light microscopy offers specificity but limited resolution (~300 nm).
- Super-resolution microscopy (SRM) significantly enhances light microscopy resolution (~10 nm).
Purpose of the Study:
- To review the integration of SRM with other microscopy techniques.
- To discuss challenges and solutions in correlative SRM.
- To highlight applications in biology and materials science.
Main Methods:
- Review of correlative super-resolution microscopy.
- Integration of SRM with light microscopy, electron microscopy, cryomicroscopy, atomic force microscopy, and spectroscopy.
- Discussion of biological studies and materials characterization.
Main Results:
- SRM overcomes conventional light microscopy resolution limits.
- Effective correlation strategies are presented for various techniques.
- Correlative SRM provides enhanced dimensional information.
Conclusions:
- Correlative SRM offers new opportunities in biological and materials research.
- Addressing current limitations will drive future advances in SRM.
- This approach expands the scope and impact of super-resolution microscopy.
Related Concept Videos
01:37Super-resolution Fluorescence Microscopy
14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
01:28Three-Dimensional Microscopy in Microbiology
946
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
946
01:16Confocal Fluorescence Microscopy
21.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.6K
01:29Two-Dimensional Microscopy in Microbiology
1.8K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.8K