Related Experiment Video
Updated: Mar 9, 2026
05:28
Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites
Published on: June 2, 2023
1.4K
A crosslinker-based identification of redox relay targets
Kazutaka Araki1, Ryo Ushioda2, Hidewo Kusano1
1Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo 135-0064, Japan.
Analytical Biochemistry
|January 4, 2017
Summary
This study identifies new cellular redox partners using a thiol-ene crosslinking method. This research advances understanding of redox regulation and homeostasis in cells.
Area of Science:
- Biochemistry
- Cellular Biology
- Molecular Biology
Background:
- Thiol-based redox control is crucial for cellular redox homeostasis.
- Cysteine thiols in oxidoreductases play a key role in these mechanisms.
- Understanding direct interactions between oxidoreductases and their targets is essential for clarifying cellular redox regulatory networks.
Purpose of the Study:
- To explore cellular redox regulatory networks by identifying direct interactions among active cysteine thiols of oxidoreductases and their targets.
- To identify new potential redox relay partners for cytosolic oxidoreductases, specifically thioredoxin (TXN) and thioredoxin domain containing 17 (TXNDC17).
Main Methods:
- Application of a recently described thiol-ene crosslinking-based strategy, the divinyl sulfone (DVSF) method.
- Utilizing classical substrate-trapping techniques in conjunction with the DVSF method.
Main Results:
- The divinyl sulfone (DVSF) method enabled the identification of novel potential redox relay partners.
- New interactions involving cytosolic thioredoxin (TXN) and thioredoxin domain containing 17 (TXNDC17) were uncovered.
Conclusions:
- The thiol-ene crosslinking strategy, exemplified by the DVSF method, is effective for identifying redox partners.
- Combining multiple methods, including substrate-trapping, enhances the understanding of cellular redox regulatory mechanisms.
Related Concept Videos
01:24Redox Reactions
59.2K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
59.2K
01:27Redox Reactions
1.3K
Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
1.3K
01:20Differential Relays
855
Differential relays are used to protect generators, buses, and transformers by comparing electrical quantities at different points. When a fault occurs, the difference in current between the two points triggers the relay to operate, opening the circuit breaker. Under normal conditions, the current entering (i1) and leaving (i2) a generator are equal. When a fault occurs, however, these currents become unequal, and the difference current flows in the relay operating coil, causing the relay to...
855
01:26Redox Titration: Other Oxidizing and Reducing Agents
1.5K
Besides iodine, other oxidizing or reducing agents can serve as titrants in redox titrations. Common oxidizing titrants include KMnO4, cerium(IV), and K2Cr2O7. The choice of oxidizing titrants depends on factors like stability, cost, analyte strength, and reaction rate between the analyte and titrant. KMnO4 is a strong oxidizing titrant that reduces from Mn(VII) to Mn(II) in a highly acidic solution, simultaneously oxidizing the analyte to a higher oxidation state. In this case, KMnO4 acts as a...
1.5K
01:23Redox Equilibria: Overview
1.7K
A reduction-oxidation reaction is commonly called a redox reaction. In a redox reaction, electrons are transferred from one species to another rather than being shared between or among atoms. The reducing agent or reductant is the species that loses electrons and gets oxidized in the process. The species that gains electrons and gets reduced in the process is the oxidizing agent or oxidant. Redox reactions are represented as two separate equations called half-reactions, where one equation...
1.7K
01:30Ladder Diagrams: Redox Equilibria
836
Ladder diagrams are useful tools for understanding redox equilibrium reactions, especially the effects of concentration changes on the electrochemical potential of the reaction. The vertical axis in the redox ladder diagrams represents the electrochemical potential, E. The area of predominance is demarcated using the Nernst equation.
Consider the Fe3+/Fe2+ half-reaction, which has a standard-state potential of +0.771 V. At potentials more positive than +0.771 V, Fe3+ predominates, whereas Fe2+...
Consider the Fe3+/Fe2+ half-reaction, which has a standard-state potential of +0.771 V. At potentials more positive than +0.771 V, Fe3+ predominates, whereas Fe2+...
836