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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Related Experiment Video

Updated: Mar 9, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
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Electron Microscopy of Fission Yeast.

J Richard McIntosh1,2, Mary K Morphew2, Thomas H Giddings1

  • 1Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347.

Cold Spring Harbor Protocols
|January 5, 2017
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Electron microscopy (EM) offers superior cell imaging resolution compared to light microscopy (LM). This study details reliable methods for fission yeast preservation and discusses EM techniques, including 3D imaging and protein localization strategies.

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Area of Science:

  • Cell Biology
  • Microscopy Techniques

Background:

  • Electron microscopy (EM) provides higher spatial resolution than light microscopy (LM).
  • EM sample preparation is time-consuming, expensive, and can introduce artifacts.
  • Understanding EM's strengths and limitations is crucial for accurate cell imaging.

Purpose of the Study:

  • To describe reliable methods for preserving fission yeast cells for electron microscopy.
  • To contrast image properties from transmission EM (TEM) and scanning EM (SEM).
  • To discuss approaches for 3D imaging and protein localization within cells using EM.

Main Methods:

  • Detailed protocols for fission yeast sample preservation for EM.
  • Acquisition and comparison of images using TEM and SEM.
  • Techniques for generating 3D TEM reconstructions.
  • Strategies for specific protein localization within cells.

Main Results:

  • Established reliable methods for fission yeast sample preservation.
  • Demonstrated the distinct imaging properties of TEM and SEM.
  • Presented approaches for 3D reconstruction and protein localization.
  • Highlighted the importance of careful image interpretation.

Conclusions:

  • Optimized EM methods enhance fission yeast cell preservation and imaging.
  • TEM and SEM offer complementary information for cell ultrastructure analysis.
  • Advanced EM techniques facilitate 3D visualization and molecular localization in cells.