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Updated: Mar 9, 2026
07:59
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
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A Novel Clerodane Diterpene from Vitex cofassus
Faradiba Abdul Rasyid1, Shuichi Fukuyoshi, Hirokazu Ando
1School of Pharmaceutical Sciences, College of Medical, Pharmaceutical and Health Sciences, Kanazawa University.
Chemical & Pharmaceutical Bulletin
|January 5, 2017
Summary
A new clerodane diterpene, 16-hydroxy-pentandralactone, and acuminolide were isolated from Vitex cofassus. Both compounds exhibited potent antiproliferative activity against human tumor cell lines, with compound 1 also inhibiting angiogenesis.
Area of Science:
- Natural Product Chemistry
- Pharmacology
- Medicinal Chemistry
Background:
- Vitex cofassus is a plant species with potential medicinal properties.
- Diterpenes are a class of natural products with diverse biological activities.
Purpose of the Study:
- To isolate and characterize new compounds from Vitex cofassus leaves.
- To evaluate the antiproliferative and anti-angiogenic activities of isolated compounds.
Main Methods:
- Methanol extraction of Vitex cofassus leaves.
- Isolation and structure elucidation of compounds using MS, NMR, and ECD.
- Antiproliferative assays against human tumor cell lines.
- Inhibition of vascular endothelial growth factor (VEGF)-stimulated human umbilical vein endothelial cells (HUVECs) proliferation assay.
Main Results:
- A new clerodane diterpene, 16-hydroxy-pentandralactone (1), and acuminolide (2) were isolated.
- Both compounds demonstrated potent antiproliferative activities (IC50: 5.4–11.4 µM) against tested cancer cell lines, including multidrug-resistant (MDR) lines.
- Compound 1 significantly inhibited VEGF-stimulated HUVEC proliferation in a dose-dependent manner.
Conclusions:
- 16-hydroxy-pentandralactone (1) is a promising candidate for an antitumor agent.
- Compound 1 also shows potential as an inhibitor of angiogenesis, suggesting a dual therapeutic approach.
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