Validation data supporting the characterization of novel copper complexes as anticancer agents.

Area of Science:

• Inorganic Chemistry
• Cancer Biology
• Molecular Pharmacology

Background:

• Copper complexes are explored for anticancer properties.
• Previous studies indicated efficacy in cervical cancer cells.
• This study extends the investigation to colon cancer models.

Purpose of the Study:

• To evaluate the anticancer activity of three novel copper(II) complexes.
• To elucidate the mechanism of action in HCT-116 colon cancer cells.
• To provide supporting data for ongoing research on copper-based anticancer agents.

Main Methods:

• Synthesis and characterization of copper(II) complexes: Cu(Sal-Gly)(phen), Cu(Sal-Gly)pheamine, Cu(Sal-Gly)phepoxy.
• Treatment of HCT-116 colon cancer cells with the synthesized complexes.
• Assessment of DNA damage markers (8-oxo-guanidine, ɣH2AX).
• Evaluation of apoptosis induction (DNA condensation, Annexin V, caspase 3/7, mitochondrial membrane potential).
• Measurement of oxidative stress levels.
• Gene expression analysis using a custom RT-qPCR array.

Main Results:

• All three copper(II) complexes induced significant DNA damage in HCT-116 cells.
• The compounds effectively triggered apoptosis through multiple pathways.
• Increased oxidative stress was observed in treated colon cancer cells.
• Gene expression data (fold changes and p-values) are provided.

Conclusions:

• The studied copper(II) complexes exhibit promising anticancer properties against colon cancer cells.
• Their mechanism of action involves inducing DNA damage, apoptosis, and oxidative stress.
• These findings complement previous research and highlight the therapeutic potential of these novel copper complexes.