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Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
Ultra-sensitive detection of kanamycin for food safety using a reduced graphene oxide-based fluorescent aptasensor
Na-Reum Ha1, In-Pil Jung1, Im-Joung La2
1Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
Abstract:
Overuse of antibiotics has caused serious problems, such as appearance of super bacteria, whose accumulation in the human body through the food chain is a concern. Kanamycin is a common antibiotic used to treat diverse infections; however, residual kanamycin can cause many side effects in humans. Thus, development of an ultra-sensitive, precise, and simple detection system for residual kanamycin in food products is urgently needed for food safety. In this study, we identified kanamycin-binding aptamers via a new screening method, and truncated variants were analyzed for optimization of the minimal sequence required for target binding. We found various aptamers with high binding affinity from 34.7 to 669 nanomolar Kdapp values with good specificity against kanamycin. Furthermore, we developed a reduced graphene oxide (RGO)-based fluorescent aptasensor for kanamycin detection. In this system, kanamycin was detected at a concentration as low as 1 pM (582.6 fg/mL). In addition, this method could detect kanamycin accurately in kanamycin-spiked blood serum and milk samples. Consequently, this simple, rapid, and sensitive kanamycin detection system with newly structural and functional analysis aptamer exhibits outstanding detection compared to previous methods and provides a new possibility for point of care testing and food safety.
Insights
Antibiotic overuse leads to superbugs. Researchers developed a highly sensitive aptasensor using aptamers and reduced graphene oxide (RGO) to detect residual kanamycin in food, ensuring safety.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Food Science
Background:
- Antibiotic overuse is a global health concern, leading to the emergence of antibiotic-resistant bacteria.
- Residual antibiotics, like kanamycin, in food products pose risks to human health through the food chain.
- Accurate and sensitive detection methods for kanamycin are crucial for ensuring food safety.
Purpose of the Study:
- To develop an ultra-sensitive, precise, and simple detection system for residual kanamycin in food products.
- To identify and optimize kanamycin-binding aptamers for enhanced detection capabilities.
- To create a novel aptasensor for rapid and reliable kanamycin quantification.
Main Methods:
- Screening and optimization of kanamycin-binding aptamers using a novel method.
- Development of a reduced graphene oxide (RGO)-based fluorescent aptasensor.
- Validation of the aptasensor using kanamycin-spiked blood serum and milk samples.
Main Results:
- Identification of high-affinity aptamers with good specificity for kanamycin.
- Development of an RGO-based fluorescent aptasensor capable of detecting kanamycin at concentrations as low as 1 pM (582.6 fg/mL).
- Accurate detection of kanamycin in spiked biological and food matrices (blood serum and milk).
Conclusions:
- The developed aptasensor offers a simple, rapid, and highly sensitive method for kanamycin detection.
- This system exhibits superior detection capabilities compared to existing methods.
- The aptasensor provides a promising tool for point-of-care testing and ensuring food safety.
