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Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
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Identification of Human Memory-Like NK Cells.

Elena I Kovalenko1, Maria A Streltsova1, Leonid M Kanevskiy1

  • 1Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russian Federation.

Current Protocols in Cytometry
|January 6, 2017
PubMed
Summary

Improved flow cytometry identifies adaptive Natural Killer (NK) cells. These memory-like NK cells, distinct from conventional types, show unique markers and respond effectively to stimuli.

Keywords:
CD57NK cell repertoireNKG2ANKG2Cadaptive NK cellsdifferentiationmemory-like NK cells

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Natural Killer (NK) cell biology understanding has advanced significantly.
  • NK cells exhibit repertoire changes during differentiation.
  • Repeating stimuli, like cytomegalovirus (CMV) infection, can lead to the accumulation of memory-like or adaptive NK cells.

Purpose of the Study:

  • To describe a method for identifying and analyzing adaptive NK cells in human peripheral blood.
  • To characterize the phenotypic and functional differences between adaptive and conventional NK cells.

Main Methods:

  • Utilized advanced flow-cytometric techniques for cell analysis.
  • Focused on identifying specific cell surface markers associated with adaptive NK cells.

Main Results:

  • Adaptive NK cells are characterized by CD57 positivity and high NKG2C expression.
  • These cells show increased KIR receptor expression and lack co-expression of NKG2A.
  • Decreased NCR3 (NKp30) expression is also a key feature of adaptive NK cells.

Conclusions:

  • The described flow-cytometric approach enables the identification of adaptive NK cell subsets using standard equipment.
  • This method contributes to a deeper understanding of adaptive NK cell populations in human peripheral blood.