Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

16.9K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
16.9K
Instrument Calibration01:12

Instrument Calibration

952
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
952
Glassware Calibration01:11

Glassware Calibration

1.7K
Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Jmjd3-mediated epigenetic regulation of inflammatory cytokine gene expression in serum amyloid A-stimulated macrophages.

Cellular signalling·2014
Same author

Overexpression of EMMPRIN isoform 2 is associated with head and neck cancer metastasis.

PloS one·2014
Same author

Designing p-type semiconductor-metal hybrid structures for improved photocatalysis.

Angewandte Chemie (International ed. in English)·2014
Same author

Chiral separation of racemic naproxen by high-performance liquid chromatography with beta-CD/SiO2 as the chiral stationary phase.

Journal of AOAC International·2014
Same author

[Anatomy of the anal canal and staging of low rectal cancer with high resolution magnetic resonance imaging].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2014
Same author

Design, synthesis and SAR study of novel trisubstituted pyrimidine amide derivatives as CCR4 antagonists.

Molecules (Basel, Switzerland)·2014
Same journal

Detection and Sorting of Extracellular Vesicles and Viruses Using nanoFACS.

Current protocols in cytometry·2020
Same journal

Live Imaging of the Lung.

Current protocols in cytometry·2020
Same journal

Small Particle Fluorescence and Light Scatter Calibration Using FCM<sub>PASS</sub> Software.

Current protocols in cytometry·2020
Same journal

Optimized Stochastic Optical Reconstruction Microscopy for Imaging Chromatin Structure in Pathological Tissue.

Current protocols in cytometry·2020
Same journal

Flow Cytometric Quantification of Granulocytic Alkaline Phosphatase Activity in Unlysed Whole Blood.

Current protocols in cytometry·2020
Same journal

Practical Guidelines for Collection, Manipulation and Inactivation of SARS-CoV-2 and COVID-19 Clinical Specimens.

Current protocols in cytometry·2020
See all related articles

Related Experiment Video

Updated: Mar 9, 2026

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
06:22

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

Published on: February 10, 2023

1.4K

Standardization, Calibration, and Control in Flow Cytometry.

Lili Wang1, Robert A Hoffman2

  • 1Biosystems and Biomaterials Division, NIST, Gaithersburg, Maryland.

Current Protocols in Cytometry
|January 6, 2017
PubMed
Summary
This summary is machine-generated.

This study explains how to use particles to calibrate flow cytometers, ensuring accurate instrument performance. It details methods for standardizing and controlling these essential instruments for reliable particle analysis.

Keywords:
ERF (equivalent number of reference fluorophores)MESF (molecules of equivalent soluble fluorochrome)calibrationcontrolparticlesresolutionsensitivitystandardization

More Related Videos

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies
10:23

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

Published on: May 19, 2023

1.3K
Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

19.0K

Related Experiment Videos

Last Updated: Mar 9, 2026

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
06:22

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

Published on: February 10, 2023

1.4K
Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies
10:23

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

Published on: May 19, 2023

1.3K
Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

19.0K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Instrumentation

Background:

  • Flow cytometers are instruments designed to measure particle characteristics.
  • Particles are commonly used for instrument calibration, control, and standardization.
  • Accurate instrument calibration is critical for reliable particle analysis.

Purpose of the Study:

  • To provide a comprehensive guide on using particles for flow cytometer calibration.
  • To clarify critical terminology and distinctions related to instrument standardization.
  • To detail practical methods for ensuring flow cytometer performance and reliability.

Main Methods:

  • Defining key terms and providing cautions for instrument calibration.
  • Describing various particle types and their application in standardization.
  • Outlining procedures for assessing optical alignment, fluorescence, and light scatter resolution.
  • Detailing methods for evaluating instrument sensitivity.

Main Results:

  • Established clear definitions and critical distinctions for flow cytometry terms.
  • Presented practical methods for standardizing and calibrating flow cytometers using particles.
  • Provided procedures to characterize instrument performance parameters.
  • Offered guidance on analyzing calibration particles.

Conclusions:

  • Particles are essential for the calibration and standardization of flow cytometers.
  • Understanding particle types and proper calibration methods ensures instrument accuracy.
  • This work provides a framework for consistent and reliable flow cytometer performance evaluation.