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Evidence for multiple lipase forms in the rabbit pancreas
P L Arzoglou1, A Konstantinidou
1Laboratory of Biochemistry, School of Chemistry, University of Thessaloniki, Greece.
Clinical Physiology and Biochemistry
|January 1, 1989
Summary
Rabbit pancreas contains two lipase forms, A and B, with distinct properties. Lipase B is recommended for use as an internal standard in lipase assays due to its characteristics.
Area of Science:
- Biochemistry
- Enzymology
Background:
- Pancreatic lipase is crucial for dietary fat digestion.
- Understanding lipase heterogeneity is important for diagnostic assays.
Purpose of the Study:
- To characterize different forms of rabbit pancreatic lipase.
- To evaluate their suitability for use in diagnostic assays.
Main Methods:
- Homogenization of rabbit pancreas.
- Separation of lipase forms using gel filtration and anion-exchange chromatography.
- Enzyme activity assays with varying salt concentrations and colipase.
Main Results:
- Two distinct lipase forms, A and B, were identified and separated.
- Lipase A showed higher affinity for emulsified triolein and partial activation by NaCl.
- Lipase B demonstrated greater colipase dependence and similarity to human pancreatitis lipase.
Conclusions:
- Rabbit pancreas possesses at least two biochemically distinct lipase forms.
- Lipase B is a suitable candidate for an internal standard in indirect lipase assays.