Related Experiment Videos
Studies on the Metabolism of Nitrogen Compounds by Prof J. W. Gorrod
1Dean of Pharmacy, Acıbadem University, Istanbul. Turkey.
Abstract:
The scientific research papers by Professor John William Gorrod who died in December 2015 are reviewed. He was one of the most important scientists in the area and investigated mainly the biological oxidation of nitrogen containing drugs and xenobiotics. In this review, more than 200 papers belong to him are evaluated, most of which involved the results from in vitro microsomal oxidative metabolism of nitrogen containing xenobiotics. These studies include the carcinogenicity of tobacco smoke constituents, aromatic amines, aminophenols, rubber and aminobiphenyls, the assay for ferriheamoglobinemia forming compounds, metabolism of aralkyl azo compounds, aromatic amines, aromatic azaheterocycles, synthesis of N-oxidative metabolites i.e. N-oxides, nitrones and hydroxylamines, mutagenicity of amines, metabolism of adenine derivatives, alicyclic amines and aromatic heterocyclic amines, in vivo and in vitro metabolism of nicotine and related tobacco alkaloids, effects of various substrates on MAO enzyme and reviews related to nicotine and N-oxidation of other nitrogen containing compounds. In the major part of these studies, the spectroscopic and chromatographic methods were developed to evaluate and determine the investigated compounds.
Insights
Professor Gorrod
Area of Science:
- Pharmacology and Toxicology
- Medicinal Chemistry
- Drug Metabolism
Background:
- Review of over 200 scientific papers by Professor John William Gorrod, a prominent scientist in biological oxidation.
- Focus on his research into the metabolism of nitrogen-containing drugs and xenobiotics, particularly through in vitro microsomal oxidation.
Purpose of the Study:
- To evaluate the extensive body of work by Professor Gorrod in the field of xenobiotic metabolism.
- To highlight his contributions to understanding the biological oxidation of nitrogen-containing compounds.
Main Methods:
- Analysis of over 200 research papers focusing on in vitro microsomal oxidative metabolism.
- Development and application of spectroscopic and chromatographic methods for compound evaluation.
- Review of studies on carcinogenicity, ferrihemoglobinemia, and mutagenicity of various nitrogen-containing compounds.
Main Results:
- Comprehensive investigation into the metabolism of diverse nitrogen-containing xenobiotics, including tobacco smoke constituents, aromatic amines, and alkaloids.
- Elucidation of N-oxidative metabolite synthesis (N-oxides, nitrones, hydroxylamines).
- Studies on the effects of substrates on Monoamine Oxidase (MAO) enzyme activity.
Conclusions:
- Professor Gorrod made significant contributions to understanding the biological oxidation and metabolism of nitrogen-containing drugs and xenobiotics.
- His research advanced the analytical methodologies used in drug metabolism studies.
- The reviewed work provides a foundational understanding of xenobiotic metabolism relevant to toxicology and pharmacology.