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Preparation of Cell Cultures and Vaccinia Virus Stocks

Catherine A Cotter1, Patricia L Earl1, Linda S Wyatt1

  • 1Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.

Current Protocols in Molecular Biology
|January 7, 2017
PubMed

Keywords:
cell culturemodified vaccinia virus Ankaravaccinia virus

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Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...